The plus and minus buttons increase and decrease the magnification of the sequence by 50%, or by 30% if the magnification is already above 50%. Annotations, translations and analysis graphs are also displayed in this viewer.Ĭontrols for zooming in and out on sequences are located at the top of the side panel, to the right of the sequence viewer. Sequences are displayed in the viewer below the document table. To remove a sequence from a list entirely, select it and click the Delete button on your keyboard, or go to Delete under the Edit menu. This will copy each sequence out of the list into a separate document, while retaining the original sequence within the list. To extract sequences from a list, select the sequence(s) you want to extract and go to Sequence → Extract Sequences from List. Note that this copies your sequences into a list and retains the original sequence documents. To existing sequences in your database into a list, or combine two lists into one, select the sequences or sequence lists you want to group and go to Sequence → Group Sequences into a List. When you import files containing multiple sequences you will be asked if you want to store those sequences in a list. Sequence lists make it easier to manage large numbers of sequences by grouping related sequences into a single document. This will create a new sequence document containing the selected sequence. To create a new sequence from an existing sequence, select the region of sequence that you want then click the Extract button above the sequence viewer, or go to Sequence → Extract Regions. Bases not in the (green) binding region will be included as a 5′ extension. If your primer contains a 5′ extension, you can specify this by setting the length of the binding region. If your sequences are oligonucleotides, choose Primer or Probe as the type. You can change this by clicking the Type option. Geneious will automatically determine whether your sequence is nucleotide or protein based on the composition of the bases you enter. Here you can paste or type in the residues for your new sequence, then enter the Name, Description and Organism for your sequence if required. New sequences can be imported from existing files as described in Importing and Exporting Data or they can be created manually by going to Sequence → New Sequence, or File → New → Sequence. Click on the first instance of a "gene" label in this feature table.Creating, Viewing and Editing Sequences Creating new sequences In the feature table, each labeled feature is hyperlinked to the sequence itself, which is at the bottom of the record. A gene may include multiple sections of coding sequences, so the same nucleotide sequence (shown in a number range) may be labeled as CDS and gene. CDS = a coding sequence, or region of nucleotides that corresponds with amino acids in a protein.Definitions of some of the feature labels can be found in the GenBank Sample Record. Scroll down the feature table of this mitochondrial DNA record. An interesting part of a Nucleotide record is the section labeled "FEATURES." Called the "feature table," this is the part that reflects scientists' annotations - notes on what biological features of interest are known about a sequence.
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